Unit

UNIT 28.4 Detection of Mycoplasma Contamination in Cell Cultures

  1. Cord C. Uphoff,
  2. Hans G. Drexler

Published Online: 14 APR 2014

DOI: 10.1002/0471142727.mb2804s106

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Uphoff, C. C. and Drexler, H. G. 2014. Detection of Mycoplasma Contamination in Cell Cultures. Current Protocols in Molecular Biology. 106:28.4:28.4.1–28.4.14.

Author Information

  1. Leibniz-Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

Publication History

  1. Published Online: 14 APR 2014

Abstract

Mycoplasma contamination of cell lines is a major problem in cell culture technology. This unit presents protocols involving either the polymerase chain reaction (PCR) or fluorescent in situ hybridization (FISH) to provide independent, fast, and sensitive techniques to monitor mycoplasma contamination in laboratory cultures. Special emphasis is placed on the integration of control reactions to prevent false-negative as well as false-positive results due to reaction inhibition or contamination and background staining, respectively. Curr. Protoc. Mol. Biol. 106:28.4.1-28.4.14. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • contamination detection;
  • FISH;
  • mycoplasmas;
  • PCR