UNIT 28.4 Detection of Mycoplasma Contamination in Cell Cultures
Published Online: 14 APR 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Uphoff, C. C. and Drexler, H. G. 2014. Detection of Mycoplasma Contamination in Cell Cultures. Current Protocols in Molecular Biology. 106:28.4:28.4.1–28.4.14.
- Published Online: 14 APR 2014
Mycoplasma contamination of cell lines is a major problem in cell culture technology. This unit presents protocols involving either the polymerase chain reaction (PCR) or fluorescent in situ hybridization (FISH) to provide independent, fast, and sensitive techniques to monitor mycoplasma contamination in laboratory cultures. Special emphasis is placed on the integration of control reactions to prevent false-negative as well as false-positive results due to reaction inhibition or contamination and background staining, respectively. Curr. Protoc. Mol. Biol. 106:28.4.1-28.4.14. © 2014 by John Wiley & Sons, Inc.
- contamination detection;