UNIT 29A.4 Tissue Collection for Systematic Phenotyping in the Mouse
Published Online: 1 OCT 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Antal, C., Teletin, M., Wendling, O., Dgheem, M., Auwerx, J. and Mark, M. 2007. Tissue Collection for Systematic Phenotyping in the Mouse. Current Protocols in Molecular Biology. 80:A:29A.4:29A.4.1–29A.4.23.
- Published Online: 1 OCT 2007
- Published Print: OCT 2007
In this unit, a procedure for post-mortem examination of mice and tissue collection is provided. This procedure is performed for post-mortem analysis of anatomical defects (necropsy) and histological analysis and/or tissue collection destined for molecular biology applications. In both cases, tissue preservation is the major issue, but the way to achieve it depends on the objective. When histological analysis is the aim, tissue preservation is achieved by rapid transfer into fixative solutions. In contrast, molecular biology applications require rapid freezing of tissue samples to preserve mRNA integrity. Consequently, performing both procedures simultaneously may be at the expense of the final product quality. Curr. Protoc. Mol. Biol. 80:29A.4.1-29A.4.23. © 2007 by John Wiley & Sons, Inc.
- tissue collection;