Unit

UNIT 29A.4 Tissue Collection for Systematic Phenotyping in the Mouse

  1. Cristina Antal1,2,
  2. Marius Teletin1,2,
  3. Olivia Wendling1,2,
  4. Mounzer Dgheem2,
  5. Johan Auwerx1,2,
  6. Manuel Mark1,2

Published Online: 1 OCT 2007

DOI: 10.1002/0471142727.mb29a04s80

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Antal, C., Teletin, M., Wendling, O., Dgheem, M., Auwerx, J. and Mark, M. 2007. Tissue Collection for Systematic Phenotyping in the Mouse. Current Protocols in Molecular Biology. 80:A:29A.4:29A.4.1–29A.4.23.

Author Information

  1. 1

    Institut Clinique de la Souris, Illkirch Cedex, France

  2. 2

    Université Louis Pasteur, Illkirch, France

Publication History

  1. Published Online: 1 OCT 2007
  2. Published Print: OCT 2007

Abstract

In this unit, a procedure for post-mortem examination of mice and tissue collection is provided. This procedure is performed for post-mortem analysis of anatomical defects (necropsy) and histological analysis and/or tissue collection destined for molecular biology applications. In both cases, tissue preservation is the major issue, but the way to achieve it depends on the objective. When histological analysis is the aim, tissue preservation is achieved by rapid transfer into fixative solutions. In contrast, molecular biology applications require rapid freezing of tissue samples to preserve mRNA integrity. Consequently, performing both procedures simultaneously may be at the expense of the final product quality. Curr. Protoc. Mol. Biol. 80:29A.4.1-29A.4.23. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • phenotyping;
  • mouse;
  • tissue collection;
  • histology;
  • necropsy