Unit

UNIT 29B.3 Evaluation of Glucose Homeostasis

  1. Sami Heikkinen1,
  2. Carmen A. Argmann2,
  3. Marie-France Champy3,
  4. Johan Auwerx4

Published Online: 1 JAN 2007

DOI: 10.1002/0471142727.mb29b03s77

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Heikkinen, S., Argmann, C. A., Champy, M.-F. and Auwerx, J. 2007. Evaluation of Glucose Homeostasis. Current Protocols in Molecular Biology. 77:B:29B.3:29B.3.1–29B.3.22.

Author Information

  1. 1

    A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland

  2. 2

    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France

  3. 3

    Institut Clinique de la Souris, Illkirch, France

  4. 4

    Institut de Genetique et de Biologie Moleculaire et Cellulaire and Institut Clinique de la Souris, Illkirch, France

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: JAN 2007

Abstract

Obesity and dyslipidemia are often found in association with insulin resistance (IR). These components combined with hypertension characterize the most common endocrine disorder in humans, the metabolic syndrome. Thus, in addition to profiling body weight evolution and lipid metabolites, glucose tolerance (a reflection of IR) and insulin sensitivity should also be considered as part of any metabolic phenotyping protocol. The ability to measure IR and glucose tolerance is important not only in the quest to fully understand the pathogenesis of the metabolic syndrome in the mouse, but also to test the effects of potential interventions. This unit presents a variety of tests used for this purpose, including direct blood glucose measurements, insulin measurement by ELISA, the homeostatic model assessment, glucose tolerance and insulin sensitivity tests, and the euglycemic clamp.

Keywords:

  • metabolic phenotyping;
  • insulin resistance;
  • glucose tolerance;
  • hyperinsulinemic-euglycemic clamp;
  • mice