UNIT 29B.4 Histopathology in Mouse Metabolic Investigations

  1. Manuel Mark1,
  2. Marius Teletin1,
  3. Cristina Antal1,
  4. Olivia Wendling1,
  5. Johan Auwerx1,
  6. Sami Heikkinen2,
  7. Konstantin Khetchoumian2,
  8. Carmen A. Argmann2,
  9. Mounzer Dgheem2

Published Online: 1 APR 2007

DOI: 10.1002/0471142727.mb29b04s78

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Mark, M., Teletin, M., Antal, C., Wendling, O., Auwerx, J., Heikkinen, S., Khetchoumian, K., Argmann, C. A. and Dgheem, M. 2007. Histopathology in Mouse Metabolic Investigations. Current Protocols in Molecular Biology. 78:B:29B.4:29B.4.1–29B.4.32.

Author Information

  1. 1

    Institut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France

  2. 2

    Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France

Publication History

  1. Published Online: 1 APR 2007
  2. Published Print: APR 2007


Due to the small size of the mouse, evaluating its clinical phenotype is sometimes problematic. In contrast, mouse models are readily accessible to post-mortem analyses at any time during the course of a disease and prior to its clinical onset. RNA, protein, and histological analyses following sacrifice represent a powerful means to identify affected cell types and molecular events underlying the altered phenotype, and therefore to understanding the signaling or metabolic pathways involved. In this unit, an overview of post-mortem analyses is provided with a strong emphasis on the principles of routine histology, including tissue fixation, processing, embedding, and staining with hematoxylin and eosin. There are also several protocols for staining with specialized histological stains used in the metabolic field to detect intracellular lipids, intracellular lipid “ghosts”, cholesterol esters, polysaccharides, mitochondria, pathological collagen deposits, and atherosclerotic plaques.


  • mouse;
  • metabolism;
  • histology;
  • fixation;
  • staining