UNIT 29B.5 Dietary Manipulation of Mouse Metabolism

  1. Jérôme N. Feige1,
  2. Marie Lagouge1,
  3. Johan Auwerx1,2,3

Published Online: 1 OCT 2008

DOI: 10.1002/0471142727.mb29b05s84

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Feige, J. N., Lagouge, M. and Auwerx, J. 2008. Dietary Manipulation of Mouse Metabolism. Current Protocols in Molecular Biology. 84:B:29B.5:29B.5.1–29B.5.12.

Author Information

  1. 1

    Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France

  2. 2

    Institut Clinique de la Souris, Illkirch, France

  3. 3

    Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008


The maintenance of metabolic homeostasis relies on the balanced intake of nutrients from food. Consequently, diet composition strongly impacts whole-body physiology. Dietary formulations with strong nutrient imbalances can lead to metabolic disorders, with lipids and simple sugars playing a prominent role. This unit describes how diet formulation can be modified to generate mouse models of human metabolic pathologies, and it details methodological procedures linked to dietary manipulations, including caloric restriction and introduction of a test compound. Curr. Protoc. Mol. Biol. 84:29B.5.1-29B.5.12. © 2008 by John Wiley & Sons, Inc.


  • metabolism;
  • diet;
  • mouse;
  • diabetes;
  • obesity