UNIT 29B.5 Dietary Manipulation of Mouse Metabolism
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Feige, J. N., Lagouge, M. and Auwerx, J. 2008. Dietary Manipulation of Mouse Metabolism. Current Protocols in Molecular Biology. 84:B:29B.5:29B.5.1–29B.5.12.
- Published Online: 1 OCT 2008
- Published Print: OCT 2008
The maintenance of metabolic homeostasis relies on the balanced intake of nutrients from food. Consequently, diet composition strongly impacts whole-body physiology. Dietary formulations with strong nutrient imbalances can lead to metabolic disorders, with lipids and simple sugars playing a prominent role. This unit describes how diet formulation can be modified to generate mouse models of human metabolic pathologies, and it details methodological procedures linked to dietary manipulations, including caloric restriction and introduction of a test compound. Curr. Protoc. Mol. Biol. 84:29B.5.1-29B.5.12. © 2008 by John Wiley & Sons, Inc.