Unit

UNIT 30.2 Targeted Metabolomics

  1. Lee D. Roberts1,
  2. Amanda L. Souza2,
  3. Robert E. Gerszten1,2,
  4. Clary B. Clish2

Published Online: 1 APR 2012

DOI: 10.1002/0471142727.mb3002s98

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Roberts, L. D., Souza, A. L., Gerszten, R. E. and Clish, C. B. 2012. Targeted Metabolomics. Current Protocols in Molecular Biology. 98:30.2:30.2.1–30.2.24.

Author Information

  1. 1

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    Broad Institute of MIT and Harvard, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 APR 2012
  2. Published Print: APR 2012

Abstract

The metabolome is the terminal downstream product of the genome and consists of the total complement of all the low-molecular-weight molecules (metabolites) in a cell, tissue, or organism. Metabolomics aims to measure a wide breadth of small molecules in the context of physiological stimuli or disease states. Metabolomics methodologies fall into two distinct groups: untargeted metabolomics, an intended comprehensive analysis of all the measurable analytes in a sample including chemical unknowns, and targeted metabolomics, the measurement of defined groups of chemically characterized and biochemically annotated metabolites. The methodologies considered in this unit focus on the processes of conducting targeted metabolomics experiments, and the advantages of this general approach are highlighted herein. This unit outlines procedures for extracting nitrogenous metabolites (including amino acids), lipids, and intermediary metabolites (including TCA cycle oxoacids) from blood plasma. Specifically, protocols are described for analyzing these metabolites using targeted metabolomics experiments based on liquid chromatography−mass spectrometry. Curr. Protoc. Mol. Biol. 98:30.2.1-30.2.24. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • targeted metabolomics;
  • liquid chromatography−mass spectrometry;
  • multiple reaction monitoring