APPENDIX 1F Safe Use of Radioisotopes
Published Online: 1 JUL 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Meisenhelder, J. and Bursik, S. 2007. Safe Use of Radioisotopes. Current Protocols in Molecular Biology. 79:1F:A.1F.1–A.1F.18.
- Published Online: 1 JUL 2007
- Published Print: JUL 2007
The pursuit of scientific knowledge has been considerably advanced by the use of biochemical molecules that incorporate radioisotopes at specific sites. The fate of these labeled molecules, and/or the radiolabeled products that result from biochemical reactions in which the parent molecule was involved, can be traced using a variety of instruments that detect radioactivity. This appendix begins with a discussion of the principles of radioactivity in order to provide the reader/user with knowledge on which to base a common sense approach to the safe use of isotopes. The characteristics of isotopes most commonly used in a molecular biology laboratory are then detailed, as well as the safety precautions and monitoring methods peculiar to each one. Detection and imaging methods used in experimental analysis are reviewed. Finally, an outline of an orderly response to a spill of radioactive material is presented. Curr. Protoc. Mol. Biol. 79:A.1F.1-A.1F.18. © 2007 by John Wiley & Sons, Inc.
- radiation safety;