APPENDIX 2 Commonly Used Reagents and Equipment

This appendix lists recipes for common reagents and also describes equipment that is typically found in a molecular biology laboratory.

Buffers and Stock Solutions

• Acid precipitation solution
  
• Ammonium acetate, 10 M
  
• BBS (BES-buffered solution), 2×
  
• CaCl₂, 1 M
  
• Denhardt solution, 100×
  
• Dithiothreitol (DTT), 1 M
  
• EDTA (ethylenediamine tetraacetic acid), 0.5 M (pH 8.0)
  
• Ethidium bromide, 10 mg/ml
  
• HBSS (Hanks balanced salt solution)
  
• HCl, 1 M
  
• HeBS (HEPES-buffered saline) solution, 2×
  
• KCl, 1 M
  
• MgCl₂, 1 M
  
• MgSO₄, 1 M
  
• MOPS buffer
  
• NaCl, 5 M
  
• NaOH, 10 M
  
• PBS (phosphate-buffered saline)
  
• Potassium acetate buffer, 0.1 M
  
• Potassium phosphate buffer, 0.1 M
  
• SDS electrophoresis buffer, 5×
  
• SED (standard enzyme diluent)
  
• Sodium acetate, 3 M
  
• Sodium acetate buffer, 0.1 M
  
• Sodium phosphate buffer, 0.1 M
  
• SSC (sodium chloride/sodium citrate), 20×
  
• STE buffer
  
• TAE (Tris/acetate/EDTA) electrophoresis buffer
  
• TBE (Tris/borate/EDTA) electrophoresis buffer
  
• TE (Tris/EDTA) buffer
  
• TEA (triethanolamine) solution
  
• TEN (Tris/EDTA/NaCl) solution
  
• TM buffer, 10×
  
• Tris-buffered saline (TBS)
  
• Tris×Cl [tris(hydroxymethyl)aminomethane], 1 M
  
• TTBS (Tween 20/TBS)