Appendix

APPENDIX 3A Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots

  1. Daniel Voytas,
  2. Ning Ke

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mba03as48

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Voytas, D. and Ke, N. 2001. Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots. Current Protocols in Molecular Biology. 48:3A:A.3A.1–A.3A.10.

Author Information

  1. Iowa State University, Ames, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999

Abstract

This unit presents procedures for visualizing and quantitating radiolabeled proteins or DNA separated by polyacrylamide gel electrophoresis (PAGE) or affixed to filter membranes. Procedures for autoradiography and densitometry are provided, but even greater sensitivity can be achieved with fluorography or by the use of intensifying screens, both of which enhance the radioactive signal. Preflashing the film is described to increase the linear measurement range for weakly radioactive samples. Sensitivity and linear ranges of measurement can also be greatly extended by using a phosphor imaging system to quickly and easily quantify radioactive samples.