Appendix

APPENDIX 3B Silanizing Glassware

  1. Brian Seed

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mba03bs28

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Seed, B. 2001. Silanizing Glassware. Current Protocols in Molecular Biology. 28:3B:A.3B.1–A.3B.2.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1994

Abstract

This unit presents a procedure to silanize (siliconize) glassware to prevent adsorption of solute to the glass surface or to increase its hydrophobicity. This is particularly important when dealing with low concentrations of particularly “sticky” solutes such as single-stranded nucleic acids or proteins.