APPENDIX 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Gallagher, S. R. 2004. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Molecular Biology. 3:3D.
- Published Online: 1 MAY 2004
- Published Print: APR 2004
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Reliable quantitation of microgram, nanogram, and even picogram amounts of DNA and RNA in solution is essential to researchers using molecular biology techniques. In addition to the traditional absorbance measurements at 260 nm, three more sensitive fluorescence techniques are presented in this appendix. These three procedures cover a range from 25 pg DNA/ml to 50 mg DNA/ml.