APPENDIX 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Published Online: 1 NOV 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Gallagher, S. R. and Desjardins, P. R. 2006. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Molecular Biology. 76:3D:A.3D.1–A.3D.21.
- Published Online: 1 NOV 2006
- Published Print: OCT 2006
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Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as well as three microvolume methods that use fiber optic technology in specialized cells or instrumentation. These procedures allow quantitation of DNA solutions ranging from 1 pg/l to 50 mg/ml.