Appendix

APPENDIX 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

  1. Sean R. Gallagher1,
  2. Philippe R. Desjardins (NanoDrop protocols)2

Published Online: 1 NOV 2006

DOI: 10.1002/0471142727.mba03ds76

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Gallagher, S. R. and Desjardins, P. R. 2006. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Molecular Biology. 76:3D:A.3D.1–A.3D.21.

Author Information

  1. 1

    UVP, Inc., Upland, California

  2. 2

    NanoDrop Technologies, Inc., Wilmington, Delaware

Publication History

  1. Published Online: 1 NOV 2006
  2. Published Print: OCT 2006

This is not the most recent version of the article. View current version (1 JAN 2011)

Abstract

Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as well as three microvolume methods that use fiber optic technology in specialized cells or instrumentation. These procedures allow quantitation of DNA solutions ranging from 1 pg/l to 50 mg/ml.

Keywords:

  • spectroscopy;
  • DNA;
  • RNA;
  • quantitation;
  • microvolume;
  • absorbance;
  • fluorescence