APPENDIX 3F Techniques for Mammalian Cell Tissue Culture
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Phelan, M. C. 2001. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Molecular Biology.
- Published Online: 1 MAY 2001
- Published Print: JUL 1996
This is not the most recent version of the article. View current version (1 MAY 2006)
The first section of this unit discusses basic principles of sterile technique, while the second section discusses medium preparation. The Basic Protocol describes subculturing of a monolayer culture grown in petri plates or flasks. Support protocols describe freezing of monolayer cells, thawing and recovery of cells, counting cells using a hemacytometer, and preparing cells for transport. An Alternate Protocol describes freezing suspension cells.