APPENDIX 3F Techniques for Mammalian Cell Tissue Culture
Published Online: 1 MAY 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Phelan, M. C. 2006. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Molecular Biology. 74:3F:A.3F.1–A.3F.18.
- Published Online: 1 MAY 2006
- Published Print: APR 2006
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
- mammalian cells;
- cell culture;