APPENDIX 3B Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 JAN 2007

DOI: 10.1002/0471142727.nsa03bs38

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Phelan, M. C. 2007. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Neuroscience. 38:3B:A.3B.1–A.3B.19.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: JAN 2007


This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.


  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer