UNIT 3.22 Isolation of Murine Natural Killer Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Reichlin, A., Iizuka, K. and Yokoyama, W. M. 2001. Isolation of Murine Natural Killer Cells. Current Protocols in Immunology. 35:IV:3.22:3.22.1–3.22.6.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
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This unit describes the isolation of natural killer (NK) cells from mouse spleen. The basic protocol describes a method for preparing a highly purified NK cell population from mouse spleen by cytotoxic depletion of contaminating cells with selected monoclonal antibodies (MAbs), complement lysis, and density-gradient centrifugation to eliminate dead cells. The advantage of this negative selection process is that the NK cells are not coated with antibody and, therefore, are not at risk of activation by antibody cross-linking. Purity can then be assessed by cell surface phenotype.