UNIT 1.3 Handling and Restraint

  1. John Donovan1,
  2. Patricia Brown2

Published Online: 1 JUL 2006

DOI: 10.1002/0471142735.im0103s73

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Donovan, J. and Brown, P. 2006. Handling and Restraint. Current Protocols in Immunology. 73:I:1.3:1.3.1–1.3.6.

Author Information

  1. 1

    Wyeth Research, Collegeville, Pennsylvania

  2. 2

    National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 JUL 2006
  2. Published Print: JUN 2006


For the safety of the handler and the animal, proper methods for handling and restraining laboratory animals should be followed. Improper handling can result in increased stress and injury to the animal. In addition, the handler risks injury from bite wounds or scratches inflicted when the animal becomes fearful or anxious. By using sure, direct movements with a determined attitude, the animal can be easily handled and restrained. Animals can be restrained either manually or in a plastic restrainer. The protocols in this unit describe handling and manual restraint of mice, rats, hamsters, and rabbits. Alternate protocols describe restraint using the plastic restrainer.


  • handling;
  • rat;
  • mouse;
  • hamster;
  • rabbit;
  • restrainer;
  • safety