UNIT 1.7 Blood Collection

  1. John Donovan1,
  2. Patricia Brown2

Published Online: 1 JUL 2006

DOI: 10.1002/0471142735.im0107s73

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Donovan, J. and Brown, P. 2006. Blood Collection. Current Protocols in Immunology. 73:II:1.7:1.7.1–1.7.9.

Author Information

  1. 1

    Wyeth Research, Collegeville, Pennsylvania

  2. 2

    National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 JUL 2006
  2. Published Print: JUN 2006


Blood is most frequently sampled for evaluation of serum antibodies or analysis of surface markers on peripheral blood cells. The protocols offered in this unit describe collection of blood from the orbital sinus or plexus of the mouse, rat, or hamster. With appropriate techniques, small amounts of blood can be obtained with little ill effect on the animal. Collection from the auricular vein or artery of the rabbit is also relatively unstressful to the animal. Bleeding procedures that should be performed on the anesthetized animal include collection from the mouse axillary plexus, cardiac puncture of the mouse, rat, hamster, or rabbit, and collection from the hamster abdominal aorta or vena cava.