UNIT 2.3 Double-Immunodiffusion Assay for Detecting Specific Antibodies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Hornbeck, P. 2001. Double-Immunodiffusion Assay for Detecting Specific Antibodies. Current Protocols in Immunology. 00:I:2.3:2.3.1–2.3.4.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
Double immunodiffusion owes its success to the unique nature of antibody-antigen interactions. When polyvalent antibodies with moderate-to-high intrinsic affinities are mixed with antigen at the right ratio (called the zone of equivalence) lattices of antibody-antigen complexes form and precipitate out of solution. When, as described in this unit, gradients of antigen and antibody are established by diffusion from adjacent wells in a bed of agar, a line of practically insoluble precipitation forms at the equivalence zone (precipitin lines).