UNIT 2.4 Production of Polyclonal Antisera

  1. Helen M. Cooper1,
  2. Yvonne Patterson2

Published Online: 1 AUG 2008

DOI: 10.1002/0471142735.im0204s82

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Cooper, H. M. and Patterson, Y. 2008. Production of Polyclonal Antisera. Current Protocols in Immunology. 82:II:2.4:2.4.1–2.4.10.

Author Information

  1. 1

    Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia

  2. 2

    University of Pennsylvania, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 AUG 2008
  2. Published Print: AUG 2008


Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large quantities of specific antibodies directed to proteins or peptides of interest is essential to the success of both basic and applied research programs. In addition, with the advent of antibody-based proteomic strategies for profiling protein expression and post-translational modification, a requirement for timely production of specific antibodies has emerged. Polyclonal antibodies derived from animals immunized with purified proteins or peptides are particularly valuable for use in the laboratory. This unit provides protocols for the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters. Curr. Protoc. Immunol. 82:2.4.1-2.4.10. © 2008 by John Wiley & Sons, Inc.


  • polyclonal antiserum;
  • adjuvant;
  • antigen;
  • immunization