Unit

UNIT 2.5 Production of Monoclonal Antibodies

  1. Wayne M. Yokoyama

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0205s03

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yokoyama, W. M. 2001. Production of Monoclonal Antibodies. Current Protocols in Immunology. 2:II:2.5.

Author Information

  1. University of California School of Medicine, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1992

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Abstract

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium (thymocyte-conditioned medium).