UNIT 2.5 Production of Monoclonal Antibodies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Yokoyama, W. M. 2001. Production of Monoclonal Antibodies. Current Protocols in Immunology. 2:II:2.5.
- Published Online: 1 MAY 2001
- Published Print: SEP 1992
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This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium (thymocyte-conditioned medium).