Unit

UNIT 2.5 Production of Monoclonal Antibodies

  1. Wayne M. Yokoyama1,
  2. Michelle Christensen2,
  3. Gary Dos Santos2,
  4. Diane Miller2,
  5. Jason Ho3,
  6. Tao Wu3,
  7. Michael Dziegelewski3,
  8. Francisca A. Neethling3

Published Online: 1 OCT 2013

DOI: 10.1002/0471142735.im0205s102

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yokoyama, W. M., Christensen, M., Santos, G. D., Miller, D., Ho, J., Wu, T., Dziegelewski, M. and Neethling, F. A. 2013. Production of Monoclonal Antibodies. Current Protocols in Immunology. 102:II:2.5:2.5.1–2.5.29.

Author Information

  1. 1

    Washington University School of Medicine, St. Louis, Missouri

  2. 2

    StemCell Technologies, Inc., Vancouver, British Columbia, Canada

  3. 3

    Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, Connecticut

Publication History

  1. Published Online: 1 OCT 2013

Abstract

This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY from StemCell Technologies). Curr. Protoc. Immunol. 102:2.5.1-2.5.29. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • monoclonal antibodies;
  • B cell hybridomas;
  • limiting dilution;
  • semi-solid methylcellulose-based medium