UNIT 2.5 Production of Monoclonal Antibodies
Published Online: 1 SEP 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Yokoyama, W. M., Christensen, M., Santos, G. D. and Miller, D. 2006. Production of Monoclonal Antibodies. Current Protocols in Immunology. 74:II:2.5:2.5.1–2.5.25.
- Published Online: 1 SEP 2006
- Published Print: AUG 2006
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This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell®-HY).
- Monoclonal antibodies;
- B cell hybridomas;
- limiting dilution semi-solid methylcellulose-based medium