Unit

UNIT 2.5 Production of Monoclonal Antibodies

  1. Wayne M. Yokoyama1,
  2. Michelle Christensen2,
  3. Gary Dos Santos2,
  4. Diane Miller2

Published Online: 1 SEP 2006

DOI: 10.1002/0471142735.im0205s74

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yokoyama, W. M., Christensen, M., Santos, G. D. and Miller, D. 2006. Production of Monoclonal Antibodies. Current Protocols in Immunology. 74:II:2.5:2.5.1–2.5.25.

Author Information

  1. 1

    Washington University School of Medicine, St. Louis, Missouri

  2. 2

    StemCell Technologies, Inc., Vancouver, British Columbia, Canada

Publication History

  1. Published Online: 1 SEP 2006
  2. Published Print: AUG 2006

This is not the most recent version of the article. View current version (1 OCT 2013)

Abstract

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell®-HY).

Keywords:

  • Monoclonal antibodies;
  • B cell hybridomas;
  • limiting dilution semi-solid methylcellulose-based medium