UNIT 2.7 Purification of Immunoglobulin G
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Andrew, S. M. and Titus, J. A. 2001. Purification of Immunoglobulin G. Current Protocols in Immunology. 21:III:2.7:2.7.1–2.7.12.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.