Unit

UNIT 2.7 Purification of Immunoglobulin G

  1. Sarah M. Andrew1,
  2. Julie A. Titus2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0207s21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Andrew, S. M. and Titus, J. A. 2001. Purification of Immunoglobulin G. Current Protocols in Immunology. 21:III:2.7:2.7.1–2.7.12.

Author Information

  1. 1

    Lancaster University, Lancaster, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.