Unit

UNIT 2.8 Fragmentation of Immunoglobulin G

  1. Sarah M. Andrew1,
  2. Julie A. Titus2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0208s21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Andrew, S. M. and Titus, J. A. 2001. Fragmentation of Immunoglobulin G. Current Protocols in Immunology. 21:III:2.8:2.8.1–2.8.10.

Author Information

  1. 1

    Lancaster University, Lancaster, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

This unit describes procedures for fragmentation of IgG to the monovalent Fab fragment using papain digestion, and to the bivalent F(ab′)2 fragment by pepsin digestion. Alternative methods of fragmentation to F(ab′)2 include use of papain that is preactivated with cysteine and use of the enzyme ficin. These alternate methods are particularly useful for mouse IgG1 antibodies.