UNIT 2.8 Fragmentation of Immunoglobulin G
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Andrew, S. M. and Titus, J. A. 2001. Fragmentation of Immunoglobulin G. Current Protocols in Immunology. 21:III:2.8:2.8.1–2.8.10.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
This unit describes procedures for fragmentation of IgG to the monovalent Fab fragment using papain digestion, and to the bivalent F(ab′)2 fragment by pepsin digestion. Alternative methods of fragmentation to F(ab′)2 include use of papain that is preactivated with cysteine and use of the enzyme ficin. These alternate methods are particularly useful for mouse IgG1 antibodies.