UNIT 2.9B Measurement of Human Serum IgD Levels
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Overed-Sayer, C. L., Mosedale, D. E., Goodall, M. and Grainger, D. J. 2009. Measurement of Human Serum IgD Levels. Current Protocols in Immunology. 85:III:2.9B:2.9B.1–2.9B.7.
- Published Online: 1 APR 2009
- Published Print: APR 2009
This unit describes an ELISA for the quantitative measurement of IgD levels in human serum. The ELISA is highly specific and sensitive, with a minimum detectable concentration of 30 pg/ml and more than 10,000-fold specificity for IgD over all other human immunoglobulins. Linear dilution characteristics enable measurement of IgD concentrations ranging over 5 orders of magnitude. These factors are vital for the IgD assay, since IgD makes up only a small proportion of the total immunoglobulins present in normal sera, and IgD serum concentrations are known to vary widely between individuals. Curr. Protoc. Immunol. 85:2.9B.1-2.9B.7. © 2009 by John Wiley & Sons, Inc.
- immunoglobulin D;