Unit

UNIT 2.9B Measurement of Human Serum IgD Levels

  1. Catherine L. Overed-Sayer1,
  2. David E. Mosedale2,
  3. Margaret Goodall3,
  4. David J. Grainger1

Published Online: 1 APR 2009

DOI: 10.1002/0471142735.im0209bs85

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Overed-Sayer, C. L., Mosedale, D. E., Goodall, M. and Grainger, D. J. 2009. Measurement of Human Serum IgD Levels. Current Protocols in Immunology. 85:III:2.9B:2.9B.1–2.9B.7.

Author Information

  1. 1

    Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom

  2. 2

    Pronostics Ltd., Babraham, Cambridge, United Kingdom

  3. 3

    Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, United Kingdom

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009

Abstract

This unit describes an ELISA for the quantitative measurement of IgD levels in human serum. The ELISA is highly specific and sensitive, with a minimum detectable concentration of 30 pg/ml and more than 10,000-fold specificity for IgD over all other human immunoglobulins. Linear dilution characteristics enable measurement of IgD concentrations ranging over 5 orders of magnitude. These factors are vital for the IgD assay, since IgD makes up only a small proportion of the total immunoglobulins present in normal sera, and IgD serum concentrations are known to vary widely between individuals. Curr. Protoc. Immunol. 85:2.9B.1-2.9B.7. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • immunoglobulin D;
  • serum;
  • ELISA