UNIT 2.10B Purification of Human IgA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Pack, T. D. 2001. Purification of Human IgA. Current Protocols in Immunology. 38:III:2.10B:2.10B.1–2.10B.7.
- Published Online: 1 MAY 2001
- Published Print: AUG 2000
This unit describes the purification of human immunoglobulin A (IgA).The main method utilizes an IgA-binding protein (IgA-bp) as an affinity reagent, similar to the IgG-binding proteins, protein A and protein G. An alternate protocol describes a method for isolating IgA1 using affinity column chromatography with Jacalin, an IgA1-specific lectin. Another alternate protocol describes IgA purification by conventional fractionation using size-exclusion chromatography.