Unit

UNIT 2.10B Purification of Human IgA

  1. Todd D. Pack

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0210bs38

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Pack, T. D. 2001. Purification of Human IgA. Current Protocols in Immunology. 38:III:2.10B:2.10B.1–2.10B.7.

Author Information

  1. Applied Biotech, San Diego, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 2000

Abstract

This unit describes the purification of human immunoglobulin A (IgA).The main method utilizes an IgA-binding protein (IgA-bp) as an affinity reagent, similar to the IgG-binding proteins, protein A and protein G. An alternate protocol describes a method for isolating IgA1 using affinity column chromatography with Jacalin, an IgA1-specific lectin. Another alternate protocol describes IgA purification by conventional fractionation using size-exclusion chromatography.