UNIT 2.12 Cloning, Expression, and Modification of Antibody V Regions
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Morrison, S. L. 2002. Cloning, Expression, and Modification of Antibody V Regions. Current Protocols in Immunology. 47:IV:2.12:2.12.1–2.12.17.
- Published Online: 1 MAY 2002
- Published Print: FEB 2002
Cloned variable (V) regions of antibodies can be expressed joined to any constant (C) region, from either the same or a different species. The resulting antibodies will have the desired associated effector functions. Chimeric antibodies obtained by joining murine V regions to human C regions should have decreased immunogenicity in humans. The process of complementarity determining region (CDR) grafting, in which the CDRs from an antibody of one species are transferred to the framework regions of another species, constitutes a further modification of this approach. The protocols presented in this unit are designed to permit PCR-based cloning of heavy and light chain V regions. This is an advanced molecular biology protocol and should be employed only by investigators who are sufficiently skilled and experienced.