UNIT 2.13 Production of Bispecific Antibodies

  1. David M. Segal1,
  2. Bert J.E.G. Bast2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0213s14

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Segal, D. M. and Bast, B. J. 2001. Production of Bispecific Antibodies. Current Protocols in Immunology. 14:IV:2.13:2.13.1–2.13.16.

Author Information

  1. 1

    National Cancer Institute, Bethesda, Maryland

  2. 2

    University Hospital, Utrecht, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


Bispecific antibodies (bsAbs) contain two different binding specificities within a single molecule and can specifically bind two different molecules together. BsAbs can be produced by chemically cross-linking purified antibodies or Fab fragments with reducible disulfide bonds or nonreducible thioether bonds, both of which are described in this unit. Protocols are also presented for producing BsAbs by fusing two antibody-producing hybridomas that can be selected for based on drug resistance, or by double labeling with fluorochromes and FACS. Support protocols describe screening and purification of bsAbs.