UNIT 2.15 Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Jordan, K. R., Crawford, F., Kappler, J. W. and Slansky, J. E. 2009. Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins. Current Protocols in Immunology. 85:IV:2.15:2.15.1–2.15.23.
- Published Online: 1 APR 2009
- Published Print: APR 2009
Methods to induce antigen-specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses, it has been more thoroughly characterized for the peptide-specific cytotoxic T cell responses. Nonspecific responses to the vaccine vehicle are controlled for by vaccinating with insect cells infected with baculoviruses encoding irrelevant antigens or no antigen. The baculovirus-infected insect cells alone are an effective immune adjuvant to elicit antigen-specific T cells. Overall, immune responses generated using this approach are similar to those generated by more conventional vaccine strategies. Curr. Protoc. Immunol. 85:2.15.1-2.15.23. © 2009 by John Wiley & Sons, Inc.
- immune responses;
- T cells;
- molecular cloning;
- insect cells