UNIT 2.15 Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins

  1. Kimberly R. Jordan1,
  2. Fran Crawford2,3,
  3. John W. Kappler1,2,3,
  4. Jill E. Slansky1

Published Online: 1 APR 2009

DOI: 10.1002/0471142735.im0215s85

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Jordan, K. R., Crawford, F., Kappler, J. W. and Slansky, J. E. 2009. Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins. Current Protocols in Immunology. 85:IV:2.15:2.15.1–2.15.23.

Author Information

  1. 1

    University of Colorado, Denver, Colorado

  2. 2

    National Jewish Health, Denver, Colorado

  3. 3

    Howard Hughes Medical Institute, Denver, Colorado

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009


Methods to induce antigen-specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses, it has been more thoroughly characterized for the peptide-specific cytotoxic T cell responses. Nonspecific responses to the vaccine vehicle are controlled for by vaccinating with insect cells infected with baculoviruses encoding irrelevant antigens or no antigen. The baculovirus-infected insect cells alone are an effective immune adjuvant to elicit antigen-specific T cells. Overall, immune responses generated using this approach are similar to those generated by more conventional vaccine strategies. Curr. Protoc. Immunol. 85:2.15.1-2.15.23. © 2009 by John Wiley & Sons, Inc.


  • vaccine;
  • immune responses;
  • peptides;
  • baculovirus;
  • T cells;
  • molecular cloning;
  • insect cells