UNIT 3.1 Isolation of Mouse Mononuclear Cells

  1. Ada M. Kruisbeek

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0301s39

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kruisbeek, A. M. 2001. Isolation of Mouse Mononuclear Cells. Current Protocols in Immunology. 39:I:3.1:3.1.1–3.1.5.

Author Information

  1. Netherlands Cancer Institute, Amsterdam, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000


This unit covers techniques for preparing lymphoid cell suspensions and removing red blood cells and dead cells. These basic manipulations are required for most of the subsequent protocols in this chapter, i.e., measuring functional responses of mouse lymphocytes.