UNIT 3.2 T Cell Enrichment By Nonadherence to Nylon
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Hathcock, K. S. 2001. T Cell Enrichment By Nonadherence to Nylon. Current Protocols in Immunology. 30:I:3.2:3.2.1–3.2.4.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
Differential adherence properties of T cells, B cells, and accessory cells such as macrophages or other APC (antigen-presenting cells) can be employed to enrich for T cells. While the basis for this adherence is not understood, either nylon wool or Sephadex G-10 can be used as column matrices to which B cells and accessory cells will more readily adhere than T cells. Such procedures offer a convenient, although imprecise, means of enriching T cells through removal of accessory and B cells; use of nylon wool is preferred if both of the latter subsets are to be removed, while Sephadex is used when the goal is primarily to remove accessory cells.