Unit

UNIT 3.2 T Cell Enrichment By Nonadherence to Nylon

  1. Karen S. Hathcock

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0302s30

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hathcock, K. S. 2001. T Cell Enrichment By Nonadherence to Nylon. Current Protocols in Immunology. 30:I:3.2:3.2.1–3.2.4.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999

Abstract

Differential adherence properties of T cells, B cells, and accessory cells such as macrophages or other APC (antigen-presenting cells) can be employed to enrich for T cells. While the basis for this adherence is not understood, either nylon wool or Sephadex G-10 can be used as column matrices to which B cells and accessory cells will more readily adhere than T cells. Such procedures offer a convenient, although imprecise, means of enriching T cells through removal of accessory and B cells; use of nylon wool is preferred if both of the latter subsets are to be removed, while Sephadex is used when the goal is primarily to remove accessory cells.