Unit

UNIT 3.7 Isolation of Dendritic Cells

  1. Kayo Inaba1,
  2. William J. Swiggard2,
  3. Ralph M. Steinman2,
  4. Nikolaus Romani3,
  5. Gerold Schuler4,
  6. Carine Brinster5

Published Online: 1 AUG 2009

DOI: 10.1002/0471142735.im0307s86

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Inaba, K., Swiggard, W. J., Steinman, R. M., Romani, N., Schuler, G. and Brinster, C. 2009. Isolation of Dendritic Cells. Current Protocols in Immunology. 86:3.7.1–3.7.19.

Author Information

  1. 1

    Kyoto University, Kyoto, Japan

  2. 2

    The Rockefeller University, New York, New York

  3. 3

    University of Innsbruck, Innsbruck, Austria

  4. 4

    University of Erlangen, Erlangen, Germany

  5. 5

    IRCL (Institut de Recherche sur le Cancer de Lille), INSERM Unité 837, Lille, France

Publication History

  1. Published Online: 1 AUG 2009
  2. Published Print: AUG 2009

Abstract

This unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen-presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function. A support protocol for collagenase digestion of splenocyte suspensions is described to increase the yield of dendritic cells. The second method involves generating large numbers of DCs from mouse bone marrow progenitor cells. In that technique, bone marrow cells are cultured in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF) to yield 5–10 × 106 cells, 60% of which express DC surface markers (e.g., B-7-2/CD86). Additional techniques for isolating DCs from mouse spleens or other mouse tissues, as well as from human tissues, are also discussed. Curr. Protoc. Immunol. 86:3.7.1-3.7.19. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • purity;
  • maturation;
  • co-stimulation;
  • antigen-presenting cells