UNIT 3.9 Early Events in B Lymphocyte Activation
Published Online: 1 NOV 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Bondada, S., Troyer, A. and Chelvarajan, R. L. 2003. Early Events in B Lymphocyte Activation. Current Protocols in Immunology. 57:II:3.9:3.9.1–3.9.14.
- Published Online: 1 NOV 2003
- Published Print: OCT 2003
B cell activation can be quantitated indirectly by assaying antibody production or directly by measuring cellular changes that occur immediately after exposure to an activation signal. This unit provides methods for the latter (direct) approach--namely, methods for quantifying early parameters of B cell activation such as increases in intracellular ionized calcium concentration [Ca2+]I, cell size, and MHC class II-antigen expression. These assays are not so much B cell specific as activation specific; as such they are a useful means of providing information about cellular events that are independent of antibody secretion. While the protocols included in this unit have been specifically designed for use with murine B cells, they can also be used with B cells obtained from other species. In addition, the method described here also applies to T cells.