UNIT 3.12 Proliferative Assays for T Cell Function
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kruisbeek, A. M., Shevach, E. and Thornton, A. M. 2004. Proliferative Assays for T Cell Function. Current Protocols in Immunology. 60:III:3.12:3.12.1–3.12.20.
- Published Online: 1 MAY 2004
- Published Print: APR 2004
This addition to unit Unavailable will describe the assays needed to evaluate CD4·CD25·T cell non-responsiveness and function. Unlike the conventional T cells described in Basic Protocol 1, CD4·CD25· cells do not proliferate to TCR stimuli alone. The conditions required to induce proliferation are described. This addition will also describe the assay in which CD4·CD25·T cells are co-cultured with conventional T cells in order to assess their suppressive function. Finally, this addition will describe the culture conditions for the activation and expansion of CD4·CD25· cells.
- T cells;
- Suppressor T cells;
- T regulatory cells;