UNIT 3.12 Proliferative Assays for T Cell Function

  1. Ada M. Kruisbeek1,
  2. Ethan Shevach2,
  3. Angela M. Thornton3

Published Online: 1 MAY 2004

DOI: 10.1002/0471142735.im0312s60

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kruisbeek, A. M., Shevach, E. and Thornton, A. M. 2004. Proliferative Assays for T Cell Function. Current Protocols in Immunology. 60:III:3.12:3.12.1–3.12.20.

Author Information

  1. 1

    Netherlands Cancer Institute, Amsterdam, The Netherlands

  2. 2

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  3. 3

    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: APR 2004


This addition to unit Unavailable will describe the assays needed to evaluate CD4·CD25·T cell non-responsiveness and function. Unlike the conventional T cells described in Basic Protocol 1, CD4·CD25· cells do not proliferate to TCR stimuli alone. The conditions required to induce proliferation are described. This addition will also describe the assay in which CD4·CD25·T cells are co-cultured with conventional T cells in order to assess their suppressive function. Finally, this addition will describe the culture conditions for the activation and expansion of CD4·CD25· cells.


  • Mouse;
  • T cells;
  • Suppressor T cells;
  • T regulatory cells;
  • Proliferation;
  • IL-2