UNIT 3.13 Production of T Cell Clones
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Fitch, F. W. and Gajewski, T. F. 2001. Production of T Cell Clones. Current Protocols in Immunology. 3:III:3.13.
- Published Online: 1 MAY 2001
- Published Print: DEC 1997
This is not the most recent version of the article. View current version (1 MAY 2006)
This unit provides methods for the derivation of specific types of T cell clones: preparation and maintenance of alloreactive murine helper T (TH) lymphocyte and cytotoxic T lymphocyte (CTL) clones using the limiting dilution technique and derivation of TH clones reactive with soluble protein antigens including a method for the selection of either TH1 or TH2 lymphocyte subsets. These two subsets of TH cells exhibit helper function in different ways and can be distinguished by the patterns of lymphokines they synthesize. Support protocols describe preparation of different types of conditioned media that can be used to enhance the growth of desired clones.