Unit

UNIT 3.13 Production of T Cell Clones

  1. Frank W. Fitch,
  2. Thomas F. Gajewski

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0313s24

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fitch, F. W. and Gajewski, T. F. 2001. Production of T Cell Clones. Current Protocols in Immunology. 3:III:3.13.

Author Information

  1. University of Chicago, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1997

This is not the most recent version of the article. View current version (1 MAY 2006)

Abstract

This unit provides methods for the derivation of specific types of T cell clones: preparation and maintenance of alloreactive murine helper T (TH) lymphocyte and cytotoxic T lymphocyte (CTL) clones using the limiting dilution technique and derivation of TH clones reactive with soluble protein antigens including a method for the selection of either TH1 or TH2 lymphocyte subsets. These two subsets of TH cells exhibit helper function in different ways and can be distinguished by the patterns of lymphokines they synthesize. Support protocols describe preparation of different types of conditioned media that can be used to enhance the growth of desired clones.