Unit

UNIT 3.13 Production of TH1 and TH2 Cell Lines and Clones

  1. Frank W. Fitch1,
  2. Thomas F. Gajewski1,
  3. Jane Hu-Li2

Published Online: 1 MAY 2006

DOI: 10.1002/0471142735.im0313s72

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fitch, F. W., Gajewski, T. F. and Hu-Li, J. 2006. Production of TH1 and TH2 Cell Lines and Clones. Current Protocols in Immunology. 72:III:3.13:3.13.1–3.13.15.

Author Information

  1. 1

    University of Chicago, Chicago, Illinois

  2. 2

    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006

Abstract

This unit describes protocols for the generation of polyclonal TH1 and TH2 cell lines from naive CD4+ T cells as well as for generation of antigen-specific cell lines from TCR-transgenic mice and antigen-specific T cell clones from primed mice. Also described are methods for the preparation and maintenance of alloreactive murine helper T (TH) lymphocyte and cytotoxic T lymphocyte (CTL) clones using the limiting dilution technique, as well as derivation of TH clones reactive with soluble protein antigens, including a method for the selection of either TH1 or TH2 lymphocyte subsets. These two subsets of TH cells exhibit helper function in different ways and can be distinguished by the patterns of cytokines they synthesize. Support protocols describe a micromanipulation method for cloning T cells and a roadmap for using protocols published elsewhere in this series to assess cytokine production by T cell clones and lines.

Keywords:

  • TH1;
  • TH2;
  • T cells;
  • IL-4;
  • IFNγ