Unit

UNIT 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes, Peyer's Patch, and Lamina Propria Cells

  1. Leo Lefrançois (IEL)1,
  2. Nils Lycke (PP and LP)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0319s17

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lefrançois, L. and Lycke, N. 2001. Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes, Peyer's Patch, and Lamina Propria Cells. Current Protocols in Immunology. 17:IV:3.19:3.19.1–3.19.16.

Author Information

  1. 1

    University of Connecticut Health Center, Farmington, Connecticut

  2. 2

    University of Göteborg, Göteborg, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1996

This is not the most recent version of the article. View current version (1 NOV 2012)

Abstract

The intestinal mucosal immune system is composed of three major lymphoid areas: the lamina propria (LP), which lies just underneath the basement membrane in the intestinal villi; (2) the intraepithelial compartment, which contains the intraepithelial lymphocytes (IEL) and is located just above the basement membrane, between the columnar epithelial cells; and (3) Peyer's patches (PP), lymphoid nodules (akin to lymph nodes) embedded in the gut wall, separated from the LP and IEL. The LP, PP, and IEL lymphoid populations form a complex, interconnected network that responds to immunological insults in the intestine. Therefore, these lymphocyte populations should be analyzed when studying the immunological status of the intestine, for example in oral immunization or in intestinal disease (including infectious disease and tumors). This unit details techniques for isolation of IEL, PP cells, and LP cells from the small intestine of the mouse.