UNIT 3.20 Isolation of Mouse Neutrophils

  1. Yi Luo,
  2. Martin E. Dorf

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0320s22

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Luo, Y. and Dorf, M. E. 2001. Isolation of Mouse Neutrophils. Current Protocols in Immunology. 22:IV:3.20:3.20.1–3.20.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1997

This is not the most recent version of the article. View current version (3 AUG 2015)


Neutrophils are widely used in research to elucidate the mechanisms of inflammation, including such processes as cell adhesion, chemotaxis, superoxide release, production of reactive nitrogen intermediates, and granule exocytosis. This unit presents a protocol for the induction of an enriched exudate of polymorphonuclear leukocytes (PMN) in the peritoneal cavity of mice. Neutrophils are then isolated using continuous gradient centrifugation. This is followed by a protocol for isolating neutrophils from peripheral blood. An alternate method for the purification of PMN from peritoneal exudate cells or peripheral blood by Histopaque density gradient centrifugation is also provided. The cell purification protocol can be modified to obtain nonelicited neutrophils from peritoneal fluid.