UNIT 3.23 Generation, Isolation, and Maintenance of Rodent Mast Cells and Mast Cell Lines
Published Online: 1 SEP 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Jensen, B. M., Swindle, E. J., Iwaki, S. and Gilfillan, A. M. 2006. Generation, Isolation, and Maintenance of Rodent Mast Cells and Mast Cell Lines. Current Protocols in Immunology. 74:IV:3.23:3.23.1–3.23.13.
- Published Online: 1 SEP 2006
- Published Print: AUG 2006
Antigen-mediated mast cell activation, with subsequent mediator release, is a major initiator of the inflammatory allergic response associated with such conditions as asthma. A comprehensive understanding of the principles involved in this process therefore is key to the development of novel therapies for the treatment of these disease states. In vitro models of mast cell function have allowed significant progress to be made in the recognition of the fundamental principles of mast cell activation via the high-affinity IgE receptor (FcɛRI) and, more recently, other receptors expressed on mast cells. In addition to human mast cells, the major cell culture systems employed to investigate these responses are rat and mouse peritoneal mast cells, mouse bone-marrow-derived mast cells, the rat basophilic leukemia cell line RBL-2H3, and the mouse MC/9 mast cell line. In this unit, we describe the protocols used for the isolation and/or culture of these cells and discuss the relative merits of their use.
- Mast Cells;
- Peritoneal mast cells;
- Mouse bone marrow-derived mast cells (BMMC);
- RBL-2H3 cells;
- MC/9 cells