UNIT 4.4 Skin Allograft Rejection

  1. Hugh I. McFarland,
  2. Amy S. Rosenberg

Published Online: 1 FEB 2009

DOI: 10.1002/0471142735.im0404s84

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

McFarland, H. I. and Rosenberg, A. S. 2009. Skin Allograft Rejection. Current Protocols in Immunology. 84:4.4:4.4.1–4.4.13.

Author Information

  1. Food and Drug Administration, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2009
  2. Published Print: FEB 2009


Skin allograft rejection is a test of the competence of T lymphocytes to mediate in vivo tissue destruction, which in turn reflects their role in critical functions such as anti-viral and tumor immunity. The tail-skin graft procedure described in this unit is useful predominantly because of the ease of preparation and resistance to ischemic (nonspecific) necrosis. Additionally, it is not necessary to sacrifice the donor mouse. However, rejection of tail-skin grafts should not be used to test for genetic homogeneity in breeding experiments or to detect minor histocompatibility (minor-H) antigens because tail skin is less sensitive than trunk skin in detecting such differences. Curr. Protoc. Immunol. 84:4.4.1-4.4.13. © 2009 by John Wiley & Sons, Inc.


  • transplantation;
  • skin;
  • allograft;
  • rejection;
  • T lymphocyte;
  • histocompatibility;
  • MHC;
  • grafting;
  • mouse