UNIT 4.4 Skin Allograft Rejection
Published Online: 1 FEB 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
McFarland, H. I. and Rosenberg, A. S. 2009. Skin Allograft Rejection. Current Protocols in Immunology. 84:4.4:4.4.1–4.4.13.
- Published Online: 1 FEB 2009
- Published Print: FEB 2009
Skin allograft rejection is a test of the competence of T lymphocytes to mediate in vivo tissue destruction, which in turn reflects their role in critical functions such as anti-viral and tumor immunity. The tail-skin graft procedure described in this unit is useful predominantly because of the ease of preparation and resistance to ischemic (nonspecific) necrosis. Additionally, it is not necessary to sacrifice the donor mouse. However, rejection of tail-skin grafts should not be used to test for genetic homogeneity in breeding experiments or to detect minor histocompatibility (minor-H) antigens because tail skin is less sensitive than trunk skin in detecting such differences. Curr. Protoc. Immunol. 84:4.4.1-4.4.13. © 2009 by John Wiley & Sons, Inc.
- T lymphocyte;