UNIT 4.5 Delayed-Type Hypersensitivity

  1. Yi Luo,
  2. Martin E. Dorf

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0405s55

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Luo, Y. and Dorf, M. E. 2001. Delayed-Type Hypersensitivity. Current Protocols in Immunology. 55:4.5:4.5.1–4.5.5.

Author Information

  1. Harvard Medical School, Boston, Massacusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1993


Delayed-type hypersensitivity (DTH) is an in vivo assay of cell-mediated immune function. DTH reactions are often divided into two phases: the sensitization phase, referring to the initial immunization with specific antigen, and the efferent or challenge phase of the DTH response, which usually follows 6 to 14 days after sensitization. This unit details the protocol for stimulating DTH responses in mice to the O-succinimide ester of the hapten 4-hydroxy-3-nitrophenyl acetyl (abbreviated NP-O-Su).