UNIT 4.5 Delayed-Type Hypersensitivity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Luo, Y. and Dorf, M. E. 2001. Delayed-Type Hypersensitivity. Current Protocols in Immunology. 55:4.5:4.5.1–4.5.5.
- Published Online: 1 MAY 2001
- Published Print: JUN 1993
Delayed-type hypersensitivity (DTH) is an in vivo assay of cell-mediated immune function. DTH reactions are often divided into two phases: the sensitization phase, referring to the initial immunization with specific antigen, and the efferent or challenge phase of the DTH response, which usually follows 6 to 14 days after sensitization. This unit details the protocol for stimulating DTH responses in mice to the O-succinimide ester of the hapten 4-hydroxy-3-nitrophenyl acetyl (abbreviated NP-O-Su).