Unit

UNIT 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras

  1. Gerald J. Spangrude

Published Online: 1 MAY 2008

DOI: 10.1002/0471142735.im0406s81

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Spangrude, G. J. 2008. Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras. Current Protocols in Immunology. 81:4.6:4.6.1–4.6.9.

Author Information

  1. University of Utah, Salt Lake City, Utah

Publication History

  1. Published Online: 1 MAY 2008
  2. Published Print: MAY 2008

Abstract

Transplantation of marrow between mouse strains congenic for CD45 after lethal irradiation establishes hematopoiesis driven by genetically marked cells in recipient animals. After several weeks, peripheral blood or primary and secondary lymphoid organs of transplant recipients can be evaluated for the presence of donor-derived cells. Two- or three-color flow cytometry can be used to identify the progeny of transplanted cells, to document their cell-surface phenotypes, and to follow development of T, B, and myeloid lineages in vivo. Curr. Protoc. Immunol. 81:4.6.1-4.6.9. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • bone marrow transplantation;
  • green fluorescent protein;
  • radiation;
  • chimerism analysis;
  • animal husbandry